xMAP® Antibody Coupling kit
Accelerate your multiplexing with xMAP® Antibody Coupling kit and accessories
The xMAP® Antibody Coupling Kit, or “AbC” kit, is a reagent kit that contains everything a researcher needs to couple an antibody to MagPlex® Microspheres.
About
Similar to an ELISA plate, MagPlex® Microspheres can be used as the substrate on which immunoassays can be performed. Unlike a traditional ELISA, however, this assay method requires much less antibody, sample, and time, with equal or better sensitivity and dynamic range.
Since each microsphere is uniquely addressable, multiple immunoassays can be performed simultaneously with the same sample in the same well. Once your desired antibody is coupled to the surface of the bead, you can begin optimizing monoplex or multiplex immunoassays to suit your needs.
Features
Kit contents
- Easy to follow coupling protocol, including guidance on how to assess coupling efficiency
- Pre-optimized reagents and buffers, including EDC3
- All required consumables (i.e. reaction tubes and transfer pipettes)
Run times
- Hands-on time = ~40mins
- Total turn-around time = ~3hrs
Kit capacity
- Small scale: up to 10 reactions of 5×106 beads/reaction or less
- Large scale: up to 4 reactions of greater then 5×106 beads/reaction
- Maximum scale is 12.5×106 beads/reaction
Number of beads that can be coupled per kit
- Maximum: 50×106 beads (4 reactions @ 12.5×106 beads/reaction)
- Minimum: 2.5×106 beads (1 reaction @ 2.5×106 beads/reaction)
Benefits
All the reagents you need in one kit
Conveniently includes all reagents and consumables necessary to couple antibodies to MagPlex® microspheres. Note: The Kit does not include microspheres or antibodies
Easy to use
Easy-to-follow coupling protocol, including guidance on how to assess coupling efficiency
Maximum bead recovery
Achieve maximum bead recovery with MagPlex® microspheres. Boost your bead recovery with MagPlex® Microspheres and magnetic separators.
Workflow
The xMAP® Antibody Coupling kit provides all the necessary reagents and protocols for activating the microspheres and for coupling of antibodies. The resulting antibody-coupled microspheres can then be used with xMAP® instruments, enabling the detection of multiple targets in a single sample.
Recommended
complementary products
MagPlex low concentration microspheres
The perfect amount of beads for assay development.
MC100XX-ID
Magnetic tube separators
Make wash steps as easy as possible with magnetic separators.
CN-0288-01
Magnetic plate separators
Make wash steps as easy as possible with magnetic separators.
CN-0269-01
xMAP® Antibody Coupling kit ordering info
Product |
Kit size |
Part Number |
|---|---|---|
xMAP® Antibody Coupling kit |
|
40-50016 |